Abstract
The present work aimed at studying the influence of different solvents (n-propyl alcohol, isopropyl alcohol and acetone) in the recovery and pre-purification of pectinases (exo-polygalacturonase-exo-PG, pectinmethylesterase-PME and pectin lyase-PMGL) from <em>Aspergillus niger</em>. The best results were obtained using isopropyl alcohol in the precipitated phase with purification factor and recovery of 2.1-fold and 20.5% for exo-PG, 7.9-fold and 77.8% for PME and 2.5-fold and 216% for PMGL enzyme. The studied method is promising in the recovery and pre-purification of pectinases enzymes.
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