Influence of deuterium-depleted water as a nutritive factor on the effectiveness of experimental chemotherapy in aging rats.

Abstract

e14683 Background: The rates of physical and chemical interactions are associated with water isotopic composition. The ability of deuterium-depleted water (DDW) to influence free radical, metabolic and regulatory processes determines the need to change the drinking diet at aging, tumor growth antitumor chemotherapy (CT). Our purpose was to study the influence of DDW on the state of aging female rats, tumor growth and the CT effectiveness. Methods: The study included 37 aging outbred white female rats (250-280 g) with transplanted sarcoma 45 (S-45) receiving chemotherapy with cyclophosphamide (CF). DDW intake (46±2 ppm, 25-30 mL a day) was started 5 weeks before S-45 transplantation. CF was administered intraperitoneally on day 10 at a dose of 50 mg/kg twice with a 5-day interval. We determined tumor volume by Shrek, deuterium concentrations in the blood plasma (NMR spectrometer JEOL JNM-ECA 400MHz) and inner organs (mass spectrometer DELTAplus H/Device, Finnigan, Germany), estrous cycle stages and types of the nonspecific adaptational reactions of organism by Garkavi-Kvakina-Ukolova (1975). Results: DDW administration before the tumor transplantation significantly balanced the stages of the estrous cycle and increased the incidences of the antistress adaptational reactions development by 1.6 times (p < 0.05); external signs of rejuvenation were observed (changes in hair state and motor activity). After S-45 transplantation, its growth prior to CT was suppressed (tumor volume 0.25±0.07 cm3 vs. 0.47±0.10 cm3 in controls, p < 0.1). DDW increased the effect of CF by 4.8 times by day 15 (0.88±0.67 cm3 vs. 4.22±1.43 cm3 with CF alone, p < 0.05). Concentrations of deuterium in the blood plasma decreased by 33%, in the inner organs (liver, kidney and heart) – by 6-14% (p < 0.05). Conclusions: DDW has an antistress, geroprotective , antitumor and CT-optimizing effect.