Abstract
The influence of β-cyclodextrin (βCD), and various βCD derivatives, on both the chemical and the physical stability of the polypeptide hormone salmon calcitonin (sCT) in aqueous solutions was investigated at elevated temperature (55°C). Also, the influence of various βCD derivatives on the enzymatic degradaton of sCT was evaluated. At pH 6, the effect of CDs on the chemical stability of sCT was negligible at CD concentrations below 5% (w/v). The only exception was the negatively charged carboxymethyl-βCD (CMβCD), which increased sCT stability. The charged CDs, i.e. 2-hydroxytrimethylammonio-propyl-βCD (TMAβCD) and CMβCD, promoted degradation in concentrated solutions of sCT at pH 6. 2-Hydroxypropyl-βCD (HPβCD) and randomly methylated βCD (RMβCD) not only inhibited aggregation, they also solubilised dimers formed in the test solutions, thereby increasing the physical stability. All the CDs tested accelerated the α-chymotryptic degradation of sCT. Maltosyl-βCD (G 2βCD), HPβCD and RMβCD showed an inhibition of leucine aminopeptidic and tryptic degradation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
