INFLUENCE OF COPPER COMPLEXES [CU(PTA)4]BF4 AND [СU(ACAC)2] ON RATS IRRADIATED WITH RADIOISOTOPE TECHNETIUM

Abstract

One of the priority tasks of modern radiobiology is the search for new, effective radioprotective compounds. In this area, metal complexes with high antioxidant activity are of particular interest. In order to study the possible favorable radioprotective effect of two copper complexes in different oxidation states, [Cu(PTA)4]BF4 and [Сu(acac)2], on the irradiated organism, we studied cytogenetic parameters in 4 groups of experimental animals. Group I included intact animals; Group II consisted of animals exposed to technetium (Tc) radioisotope, which were intraperitoneally injected with an isotope with an activity of 4.8 mCi in a volume of 2 mL - "pure irradiation"; Group III consisted of animals that were intraperitoneally injected with the copper(I) complex [Cu(PTA)4]BF4 at a dose of 50 mg/kg in a volume of 2 mL one hour before the administration of the Tc isotope (“irradiation + [Cu(PTA)4]BF4”). Group IV included animals that received the copper(II) complex [Сu(acac)2] before irradiation. We studied survival and cytogenetic parameters, determined the mitotic index, chromosomal aberrations and the percentage of polyploid cells. The survival rate of group II was 40%. In the group of intact animals, as well as in groups III and IV the survival rate was 100%. The dynamics of survival was described by regression equations, which make it possible, by means of extrapolation, to determine the change in the percentage of survival in the long term of the experiment and to predict the further outcome of the experiment. Investigating cytogenetic indicators, for all 3 indicators, a significant difference was found between intact and irradiated animals, i.e. these indicators can be considered as markers of technetium isotope exposure. In terms of proliferative activity, a significant difference was found in irradiated animals compared with groups III and IV, which indicates the radioprotective property of both the copper compounds. As for the [Cu(acac)2], when it was used in group IV and compared with others, we obtained a pronounced significant difference from the group with “pure irradiation” in all studied cytogenetic parameters. It was found that the copper complexes [Cu(PTA)4]BF4 and [Cu(acac)2] have the ability to prevent or mitigate the effect of ionizing radiation on an animal's body. Based on cytogenetic parameters, it can be concluded that these compounds promote reparative processes in the bone marrow cells of irradiated animals. Multiregression analysis of cytogenetic parameters confirmed the highest efficiency of the copper (II) complex [Cu(acac)2]. The results of the research indicate the need to continue work in the direction of searching for agents that have a therapeutic effect in radiation injuries.