INFLUENCE OF COPPER COMPLEXES [CU(PTA)4 [BF4 ] AND CU(II)2 (3,5-DIPS)4 (H2 O)3 ON THE ORGANISM OF RATS IRRADIATED WITH RADIOISOTOPE TECHNETIUM

Abstract

One of the priority tasks of modern radiobiology is the search for new, effective radioprotective compounds. In this area, metal-organic complexes with high antioxidant activity are of particular interest. In order to study the possible beneficial radioprotective effect of copper complex compounds [Cu(PTA)4 [BF4 ] and Cu(II)2 (3,5-DIPS)4 (H2 O)3 on an irradiated organism, we studied cytogenetic parameters in 4 groups of experimental animals: intact animals, animals exposed to the radioisotope technetium (Tc) — "pure irradiation", animals with "irradiation + compound [Cu(PTA)4 [BF4 ]", and animals with "irradiation + compound [Cu(II)2 ( 3,5-DIPS)4 (H2 O)3 ". The survival rate and cytogenetic parameters were studied: mitotic index (MI), chromosomal aberrations (CA) and polyploid cells (PC) in the bone marrow cells of the femur. The survival rate in the first and third groups was 100%, in the second group — 40%, and in the fourth — 80%. The dynamics of survival was described by regression curves and equations, which make it possible, using extrapolation, to determine the change in the percentage of survival in the long term of the experiment and to predict the further outcome of the experiment. When analyzing the results in 4 groups of animals, we found a significant difference in cytogenetic parameters between these groups. Thus, for all 3 indicators, a significant difference is observed between intact and irradiated animals, i.e. these parameters can be considered as markers of Tc exposure. In terms of the mitotic index (proliferative activity), a significant difference was found in the irradiated compared with the groups: "irradiation + [Cu(PTA)4 [BF4 ]" and "irradiation + Cu(II)2 (3,5-DIPS)4 (H2 O)3 ", which indicates the radioprotective property of both compounds. By the number of polyploid cells, a significant difference was found between the groups: "pure irradiation" and "irradiation + Cu(II)2 (3,5-DIPS)4 (H2 O)3 ", which also proves the beneficial effect of this compound. Multiregression analysis of cytogenetic parameters along with standard statistical methods confirmed the highest efficiency of [Cu(PTA)4 [BF4] relative to Cu(II)2 (3,5-DIPS)4 (H2 O)3 .