INFLUENCE OF COMBINED PHARMACOPROPHYLAXIS OF INFECTIOUS DISEASES ON THE CONDITION OF THE GASTROINTESTINAL TRACT OF LABORATORY RATS

Abstract

The aim of the work was studying the possibility of combined pharmacoprophylaxis of infectious diseases during the use of antibiotics in laboratory animals by applying a complex of medicines, including vitamins and minerals. Methods. The experiment was performed on 16 rats aged 1.5 months: 8 – intact, 8 – pharmacoprophylaxis (Baytril Bayer 10 mg/kg; ascorbic acid 2 g/l; glucose solution 50 g/l; Chiktonic 1 ml/l; Baycox 3 ml/l) within 24 days. For biochemical studies, the liver, mucous membranes of the stomach, small and large intestine were isolated, in which indicators of inflammation (activity of elastase and acid phosphatase), the state of non-specific antimicrobial protection (activity of lysozyme), indicator of microbial insemination (activity of urease) were determined. Results. Pharmacoprophylaxis according to the scheme in the mucous membranes of the digestive tract of rats led to an increase in markers of inflammation (activity of acid phosphatase and elastase), development of dysbiosis (increase in urease activity against the background of decrease in lysozyme activity). More significant changes were registered in the mucous membrane of the large intestine. In the liver of rats, an increase in the activity of elastase, acid phosphatase and urease was established along with decrease in the activity of lysozyme, which indicates the presence of inflammation and a violation of the antitoxic and antimicrobial functions of the liver. Conclusion. The study showed the insufficiency of the use of vitamin-mineral complex, ascorbic acid and glucose as protective components of the digestive tract against the negative effects of antibiotics. This dictates the need to find more effective protective agents, capable of preventing the negative effects of antibiotics.