Abstract

 baumannii is an aerobic gram negative coccobacilli, it is considered multidrug resistance pathogen (MDR) and causes several infections that are difficult to treat. This study is aims to employ physical methods in sterilization and inactivation of A. baumannii, as an alternative way to reduce the using of drugs and antibiotics.
 
 Cold Atmospheric Plasma was generated by one electrode at 20KV, 4 power supply and distance between electrode and sample was fixed on 1mm. A. baumannii (ATCC 19704 and HHR1) were exposed to Dielectric Barrier Discharge type of Cold Atmospheric Plasma (DBD-CAP) for several periods of time (15, 30, 45, and 60 sec.) . After sterilization test, several methods were done to analyze the effect of DBD-CAP on bacterial morphology, proteins and DNA. Change in morphology was assessed by cover slid method. Damaged DNA was investigated by PCR technique, and DNA sequencing. The impact of DBD-CAP on the entity of proteins was detected by SDS-PAGE. The observed inactivation of bacterial colony on agar plates has been quantified by measuring the inactivation diameter.
 The important conclusion that HHR1 more resistance to DBD-CAP than ATCC 17904 because it is more virulence than standard strain; thus, the growth of both strains is largely affected by plasma and this influence is increased by increasing the time of exposure, also the plasma affects the DNA especially on standard strain as it is explained in sequencing result, so it causes more deletion in DNA sequence. In addition, plasma also has been showed to damage proteins and morphology thus, the bacterial cells transform from cocco-bacillus to bacillus.
Highlights
Acinetobacter baumannii is an aerobic, nonfermented Gram-negative coccobacilli, it is grown well on solid media with smooth grayish white colonies
This study aims to employ Cold Atmospheric Plasma (CAP) in treatment of A. baumannii infections to reduce the using of drugs and antibiotics
In A. baumannii ATCC17904, the inactivation gap was increased from 2.5 cm to 4 cm after 60 sec, while the HHR1isolated was less inhibited after 60 sec exposure time (Table 1)
Summary
Acinetobacter baumannii is an aerobic, nonfermented Gram-negative coccobacilli, it is grown well on solid media with smooth grayish white colonies It is identified as one of the main multidrug resistance (MDR) organisms, and it has been considered as a very important pathogen that causes several hospital and community infections, such as ventilator and community acquired pneumonia, meningitis, wound and trauma infections, and septicemia [1, 2]. Cold Atmospheric Plasma has been employed in several sides like, polymerization [7], sterilization/inactivation of microorganisms, this is more essential part since it is participate in keeping life for human beings It has been depended on either physical or chemical process that destroys or reduces microorganisms, or Baghdad Science Journal both [8,9] and CAP has been proposed as a therapeutic method to treat cancer [10]. The study focuses on the effect of CAP on OmpA gene of A. baumannii
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