Abstract
Three-month-old Carrizo citrange (hybrid of Citrus sinensis L. Osbeck×Poncirus trifoliata Blanco) seedlings were grown in controlled environment chambers in pots of fine sand. Plants were irrigated with either non-saline or saline solutions over a 3-week period. After these treatments, plants were transferred to vessels containing a 5 m m15NO3K (96% atom excess15N) solution, and transpiration as well as concentration of15N and Cl−in roots, stem and leaves were measured after 24 h. Transpiration and15NO3−uptake rates were inhibited after exposure to NaCl and the concentration of salt pre-treatment determined the intensity of this inhibitory effect. To determine the effect of transpiration on NO3−absorption, net15NO3−uptake rate was measured in salt stressed and non-stressed plants exposed to different light intensities or relative humidities and also in detached roots. Reduction in NO3−uptake was more closely related to Cl−antagonism from salt stress than to reduced transpiration rate.
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