INFLUENCE OF BOVINE BIOMATERIAL MIXED OR NOT WITH FIBRIN RICH PLASMA ON HUMAN EXFOLIATED DECIDUOUS TEETH

Abstract

<h3>Background & Aim</h3> The use of mesenchymal stem cells associated with different scaffolds has been the goal in recent regenerative and tissue engineering. The aim of the present study was to analyze the viability, cytotoxicity and proliferation of stem cells from human exfoliated deciduous teeth (SHEDs) in the presence of a bovine biomaterial associated or not with platelet-rich fibrin (PRF). <h3>Methods, Results & Conclusion</h3> The studied groups were divided as S (SHEDs only, control group); SB (SHEDs + biomaterial); SBP (SHEDs + biomaterial + PRF). In 24-well plates, cells were incubated for 24, 48, and 72 hours. The performed tests were neutral red, MTT and crystal violet, besides scanning electron microscopy (SEM) to assess cell ultramorphology. Data were submitted to statistical analysis by two-way ANOVA, with a significance level of 5%. Regarding viability, cytotoxicity and proliferation, the SBP group presented increased results, compared to SB and S groups, with statistical significance. SHEDs under the bovine biomaterial influence presented higher viability and proliferative with lower cytotoxicity characteristics, especially when associated with PRF. Cells under biomaterials influence were sparse, with fibroblast-like morphology, besides the presentation of filopodia and granules at the membrane surface. In the presence of a bone bovine biomaterial, the PRF seemed to enhance the metabolism of SHEDs in culture, demonstrating to be an active functional scaffold.