Abstract
It is known that a charge is generated in water and protein solutions upon their motion; this phenomenon is also observed in analytical systems for atomic force microscopy (AFM)-based fishing. At that, the efficiency of protein fishing correlates with the value of charge, generated upon the motion of the analyzed solution. Earlier, we demonstrated that a pulsed electric field can well be used for the enhancement of the efficiency of AFM-based fishing of low-abundant protein. In this paper, we have demonstrated the influence of a pulsed electric field on the stimulation of the electric charge generation in a solution of low-abundant proteins observed in the injector part of an AFM-based fishing system at 23 °C and 38 °C. Taking this effect into account is important for the development of novel highly sensitive flow-based diagnostic systems, as well as for the development of models describing the influence of a pulsed electric field on pathological processes in the body, hemodynamics, and physicochemical properties of solutions.
Highlights
Development of novel approaches for the detection of low-abundant proteins is of high importance for highly sensitive proteomics
To study the effect of a pulsed electric field on the generation of charge in water and in protein solutions, we have used a system employing the atomic force microscopy (AFM)-based fishing device described in detail elsewhere [5]
The data obtained in our present study describe the stimulating effect of an external pulsed electric field on the generation of charge in water and protein solution upon their motion through the injection part of an AFM-based fishing system
Summary
Development of novel approaches for the detection of low-abundant proteins is of high importance for highly sensitive proteomics. We observed the occurrence of this effect in atomic force microscopy (AFM)-based fishing systems for the detection of low-abundant proteins upon flowing of an analyte solution through an injector into a measuring cell of this system [2,3,4,5]. In the paper [2], it was reported that the efficiency of protein detection correlates with the amount of charge (in nanoCoulombs, nC). Studying this effect is very important for modeling hemodynamics and mechanisms of functioning of living systems. The stimulating effect of a pulsed electric field on the efficiency of protein fishing in an AFM-based fishing system was demonstrated earlier with the example of human cytochrome b5 protein [5]
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