Influence of a new wound-healing ointment on morphogenesis of full-thickness excision wounds in healthy rats and rats with diabetic state.

Abstract

Background. Impaired wound healing is a complication of diabetic state and is a serious medical and social problem. It occurs as a consequence of disequilibrium in reactive oxygen species (ROS) and antioxidant defense system. Therefore local treatment with antioxidants is promising in this type of pathology. Objective. The aim of this study was morphological evaluation of the influence of a new wound-healing ointment containing licorice extract, coenzyme Q10 and dihydroquercetin on the model of natural and delayed wounds in rats. Methods. For morphological evaluation on Day 6 and 12 we took specimens of tissues with wound and surrounding intact skin. Prepared histological slides and stained with haematoxylin, eosin and picrofuchsin by Van Gieson. Results. It was noted that process of excision wound healing in rats with diabetic state had prolonged inflammatory phase. Thus, on the Day 6 of observation during wound-healing in a physiologically healthy rats fibrinoid-necrotic layer in the surface layers of the defect was quite dense, with a limited number of leukocyte cells. Young newly formed granulation tissue in the bottom areas also purified from neutrophil leukocytes. Inflammatory reaction in the boundary to the defect areas of skin was extinguished. And in rats with diabetic state same fibrinoid-necrotic layer was markedly attenuated, full of polymorphonuclear leukocytes, granulation cells also contained many white blood cells in the boundary areas of healthy skin observed exocytosis of epithelial layer, a marked inflammation and destruction of the dermis. All this led to decrease in speed of development and maturation of granulation tissue on the 12th day of observation in these animals compared with physiologically healthy rats. Conclusion. The results showed that the studied ointment optimizes reparative tissue regeneration in the healing of skin excision wounds in rats with pre-modeled diabetogenic state. Nature of reparative processes in these rats generally followed that of the physiologically healthy rats.