Abstract
Introduction GRP78 has a critical role in the attenuation of cellular stress and recovery from injury. It is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER) and is induced in times of ER stress. Russell bodies (RB) are dilated ER cisternae containing condensed immunoglobulins and are thought to arise from failure of ER quality control mechanisms. Purpose To investigate the relationship between Russell bodies and ER stress in periodontal inflammation. Methods Formalin fixed paraffin embedded (FFPE) specimens were retrieved and categorised into three groups: inflamed period-ontal tissues with RB (Group 1), inflamed tissues without RB (Group 2), and lightly inflamed control tissue (Group 3). Serial sections were prepared and stained with H&E, PAS, methyl green pyronin and anti-GRP78. The number of RB and the distribution and types of cells staining positively with GRP78 were analysed. Results Of the inflammatory cells in Groups 1 and 2 which were GRP78+, most were plasma cells. In the less inflamed Group 3 tissues lymphocytes were also GRP78+. Inflamed tissues had statistically significant more apoptotic/degenerate bodies than control tissues. There was no statistically significant difference between GRP78 staining and the presence or absence of RB in inflamed tissues and control tissues. Conclusion Inflammation, rather than RB, correlated positively with GRP78 immunostaining.
Published Version
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