Abstract
Monkey kidney tissue culture cells infected with type 1 poliovirus contain not only mature virions, but also a considerable amount of unbound or free viral RNA. The presence of such an RNA in virus harvests was shown by several methods such as: (1) facilitation and RNase sensitivity; (2) stability to elevated temperatures (52°); (3) stability of infection in presence of polio antiserum; and (4) chromatography on Sephadex G200. Furthermore the density of this free RNA was determined and compared with the density of RNA prepared from virions (phenol method). Both RNA's had a density of 1.63 g/ml in Cs 2SO 4, suggesting a single-stranded structure for the free viral RNA. The appearance of free viral RNA in the growth cycle of poliovirus was studied. The results indicated that maximum yield of free viral RNA was obtained approximately 10–15 hours after the maximal amount of virions was synthesized.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
