Abstract
Background: Some fish viruses, such as piscine orthoreovirus and infectious salmon anemia virus, target red blood cells (RBCs), replicate inside them and induce an immune response. However, the roles of RBCs in the context of infectious pancreatic necrosis virus (IPNV) infection have not been studied yet.Methods: Ex vivo rainbow trout RBCs were obtained from peripheral blood, Ficoll purified and exposed to IPNV in order to analyze infectivity and immune response using RT-qPCR, immune fluorescence imaging, flow cytometry and western-blotting techniques.Results: IPNV could not infect RBCs; however, IPNV increased the expression of the INF1-related genesifn-1,pkrandmxgenes. Moreover, conditioned media from IPNV-exposed RBCs conferred protection against IPNV infection in CHSE-214 fish cell line.Conclusions: Despite not being infected, rainbow trout RBCs could respond to IPNV with increased expression of antiviral genes. Fish RBCs could be considered as mediators of the antiviral response and therefore targets of new strategies against fish viral infections. Further research is ongoing to completely understand the molecular mechanism that triggers this antiviral response in rainbow trout RBCs.
Highlights
Fish viral infections cause significant losses in aquaculture
infectious pancreatic necrosis virus (IPNV) did not infect rainbow trout red blood cells (RBCs) To evaluate the infectivity of IPNV in rainbow trout RBCs, RBCs were exposed to IPNV at MOI 0.5 and the viral RNA was evaluated by RT-qPCR in the cell pellet at different times post-exposure
IPNV exposure increased the expression of interferonrelated antiviral genes and proteins in rainbow trout RBCs To determine if IPNV would induce an antiviral response in RBCs, RT-qPCR analysis of IFN-related antiviral genes was performed for IPNV-exposed RBCs
Summary
Fish viral infections cause significant losses in aquaculture. Infectious pancreatic necrosis (IPN) is a highly contagious viral disease with a high impact on salmonid aquaculture industry. IPNV belongs to the Aquabirnavirus genus within the Birnaviridae family Viruses of this family are nonenveloped particles with a double stranded RNA genome. Aquabirnavirus genus has been written instead of Aquabirnaviridae genera Some fish viruses, such as piscine orthoreovirus and infectious salmon anemia virus, target red blood cells (RBCs), replicate inside them and induce an immune response. Conclusions: Despite not being infected, rainbow trout RBCs could respond to IPNV with increased expression of antiviral genes. Further research is ongoing to completely understand the molecular mechanism that triggers this antiviral response in rainbow trout RBCs. Remove “highly” I suggest “the roles of RBCs in birnavirus....”. “Despite not being infected, trout RBCs respond to IPNV with increased expression of antiviral genes.”. I suggest “the roles of RBCs in birnavirus....” Authors response: It has been corrected
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