Abstract
Hyptis leucocephala is an aromatic herb endemic to the Brazilian semiarid region, with antimicrobial, antifungal,cytotoxic, anti-inflammatory and anti-HIV properties. In vitro cultivation makes the production of high genetic andsanitary quality cuttings possible for this species, allowing its sustainable exploitation. In the present study, the effect of 6-benzilaminopurine (BAP) and naftalenoacetic acid (NAA) on callus induction in leaf segments of this species anddifferent organic additives to WPM (Wood Plant Medium) for the callus induction was evaluated. The combination ofBAP and NAA allowed the induction of compact and undifferentiated calluses. The highest rate of callus formation andgrowth was obtained with the combination of 8.88 μM BAP and 21.48 μM NAA. Supplementing the culture medium withcoconut water, malt extract and yeast extract did not induce friable calluses. The levels of total sugars, reducing sugars,protein, and amino acids were reduced in calluses maintained in culture medium supplemented with coconut water, maltextract and yeast extract.
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