In vitro Shoot Multiplication of Curcuma xanthorrhiza with Coconut Water and Banana Extract Nutrient

Abstract

Curcuma xanthorrhiza is a medicinal plant, commonly used for spices, growth by rhizome, which is as its main product. Its rhizome demand both quantity and quality are increasing every year. Tissue culture techniques can achieve the efforts for providing plant materials massively in the short-term time with free from pest. In vitro, multiplication is an alternative production method. However, it is still limited by high chemical cost and plant growth regulators. Moreover, various natural materials can be used as a substitute for plant growth regulators, such as coconut water extract and various organic materials. The purpose of this study is to assess the response of Curcuma xanthorrhiza explants with coconut water, and Cavendish bananas extract by in vitro. The experiments conducted in February until May 2016 in the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Sebelas Maret University Surakarta. A completely randomized design was applied with two factors: the first factor was the four concentrations Cavendish banana fruit extract: 0 g/l, 50 g/l, 100 g/l and 150 g/l. The second factor were the 4 concentrations coconut water: 0 ml/l, 100 ml/l, 150 ml/l, and 200 ml/l. The observed variables were the period of shoots to appear, the period of leaves to appear and the period of roots to appear, the number of shots, the number of leaves, the number of roots, the height of shoots, the length of leaves and the length of roots. Observed data analyzed by descriptive and variance analysis continued with Duncan Multiple Range Test (DMRT). Research results showed that there were some effects on coconut water and Cavendish banana extract treatment toward the average time of shoots appear, number of shoots, number of leaves and length of leaves. The best treatment for this research is a combination of coconut water at 150 ml/l and 100 g Cavendish banana extract.