Induksi Tunas pada Kotiledon dan Hipokotil Tanaman Jarak Pagar (Jatropha curcas L.) melalui Organogenesis Tak Langsung

Abstract

<p>Propagation through tissue culture of plant<br />species with rich secondary metabolites such as Jatropha<br />curcas L. is difficult to obtain. However, once established, it<br />can be used as one of the alternatives to supply uniform<br />propagules. The effects of auxin and cytokinin on the<br />regulation of de novo woody plants shoot development have<br />been studied through shoot induction, differentiation and<br />development. The objective of this research was to identify<br />explant and suitable culture media for in vitro shoot induction<br />through indirect organogenesis. Factorial experiment<br />was arranged in a completely randomized design, replicated<br />20 times. The first factor was explants, i.e. cotyledons and<br />hypocotyls. The second factor was MS media containing<br />combination of plant growth regulator IAA (0, 0.05, and 0.1<br />mg/l) and BAP (0, 1.0, 2.0, 3.0 mg/l). The results of the<br />experiment showed that the fastest callus initiation was<br />achieved by MS + IAA 0.1 mg/l, i.e. 9.5 days after explants<br />were cultured. Shoots with leaves can be induced from both<br />cotyledons and hypocotyls. However, hypocotyls gave more<br />shoots and leaves than cotyledons when cultured in MS +<br />IAA 0.1 mg/l + BAP 3.0 mg/l. Shoots obtain from hypocotyls<br />and cotyledons were successfully rooted in MS medium<br />without any growth regulator.</p>