Abstract
The research of callus induction in potato (S. tuberosum L.) from Dombu Village with indole-3-acetic-acid (IAA) was conducted from Januari to April 2019 in Laboratory of Tissue Culture, Biology Department, Faculty of Mathematics and Natural Science, Tadulako University. The research was aimed to determine of concentration IAA of the best to induce callus. This study was designed based on Completely Randomized Design (CRD) with 5 treatments, 3 replications and 3 explants on each unit. The treatments were P1= MS + 0 ppm, P2= MS + 0.5 ppm, P3= MS + 1.0 ppm, P4= MS + 2.0 ppm, P5= MS + 3.0 ppm. The results showed that the best and the efficient treatments for induction of callus treatment was P4= MS + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture.
Highlights
Latar Belakang Kentang merupakan tanaman sayuran semusim berumur pendek (Mailangkay dkk., 2012; Hidayah dkk., 2017) yang tumbuh mencapai 100 cm dan menghasilkan umbi yang kaya akan pati (Alsulaiman et al, 2011) serta merupakan makanan terpenting dunia ke empat sesudah padi, gandum dan jagung (Wang et al, 2011; Faruk et al, 2014; Rai dkk.,2015; Munira et al, 2015)
The results showed that the best and the efficient treatments for induction of callus treatment was P4= Murashige dan Skoog (MS) + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture
Induksi kalus dari hipokotil alfafa (Mediacago sativa L.) secara in vitro dengan penambahan benzyl amino purin (BAP) dan α-naphtalene acetid acid (NAA)
Summary
Latar Belakang Kentang merupakan tanaman sayuran semusim berumur pendek (Mailangkay dkk., 2012; Hidayah dkk., 2017) yang tumbuh mencapai 100 cm dan menghasilkan umbi yang kaya akan pati (Alsulaiman et al, 2011) serta merupakan makanan terpenting dunia ke empat sesudah padi, gandum dan jagung (Wang et al, 2011; Faruk et al, 2014; Rai dkk.,2015; Munira et al, 2015). Kultur kalus bertujuan untuk memperoleh kalus dari eksplan yang diisolasi dan ditumbuhkan secara invitro. Penelitian ini menginduksi kalus dari eksplan nodus kentang dengan menggunakan hormon IAA. Nodus berisi mata tunas yang bersifat meristematik dan IAA dikenal sebagai zat pengatur tumbuh auksin yang dapat mempercepat pertumbuhan kalus.
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