Abstract
Rivai RR, Helmanto H. 2015. Callus Induction of Chrysantemum indicum for increasing genetic diversity from somatic cell. Pros Sem Nas Masy Biodiv Indon 1 (1): 167-170. Chrysantemum indicum L. is cut flowers that have been mass-produced in Indonesia. Somaclonal variation of in vitro culture can be used as an alternative way for plant breeding. Plant growth regulator is a factor for callus induction. Callus can be produced from various explants such as leaf, hypocotyl, cotyledon, stem, zygotic embryo and other plant parts that are cultured in medium containing auxin compounds especially 2.4-dichlorophenox acetic acid (2,4-D). The objective of this research was to obtain the best concentration of 2.4-D within Murashige and Skoog (MS) basal medium for callus induction of Chrysantemum indicum L. from different types of explants. The results showed that MS medium combined with 3 mg L-1 2,4-D was the best treatment for inducing callus from the leaf. However, MS medium combined with 1 or 2 mg L-1 2,4-D were the best treatments for inducing callus from internode.
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