Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica 'Jugana'.

Júlia Hunková,Alena Gajdošová,Monika Szabóová

doi:10.3390/plants10061155

Abstract

The aim of this work was to assess the regeneration capacity of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica cv. ‘Jugana’ from different types of explants under various hormonal treatments. The whole leaves, petioles, and internodal segments of in vitro plants were examined as explants. Several plant growth regulators (cytokinins and auxins) were evaluated for their ability to induce adventitious regeneration. Direct and indirect organogenesis was achieved under certain culture conditions in both species. The frequency of shoot regeneration was strongly dependent on concentrations of plant growth regulators in the induction media (L. kamtschatica ‘Jugana’) or concentrations of plant growth regulators in the induction media and type of explant (A. alnifolia var. cusickii). Results showed that leaves were not suitable explants for A. alnifolia var. cusickii. Both species were able to regenerate shoots from internodal segments and petioles. The highest induction of shoots was obtained on Murashige and Skoog (MS) medium enriched with 2 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-butyric acid (IBA) for Amelanchier alnifolia and with 1 mg/L TDZ and 0.2 mg/L indole-3-acetic acid (IAA) for L. kamtschatica ‘Jugana’. Obtained adventitious shoots were further proliferated in order to investigate their multiplication capacity. The multiplication of shoots was successful in all cultivars, with the best results reported in A. alnifolia var. cusickii (7.07 shoots/explant on average).

Highlights

Berries belong to the group of economically important crops mostly due to their benefits on human health [1]
Testing of explant type showed that A. alnifolia var. cusickii leaves of in vitro plants
Testing of explant type showed that A. alnifolia var. cusickii leaves of in vitro plants were not suitable for adventitious shoot induction

Summary

Introduction

Berries belong to the group of economically important crops mostly due to their benefits on human health [1]. Saskatoon fruit could be a suitable supplement for modern human nutrition, as the berries are rich source of polyphenols, flavonoids (gallic acid, rutin, quercetin), and anthocyanines [7]. Lonicera kamschatica (honeysuckle) is a deciduous shrub native to Russia and Japan. To saskatoon, it is frost- and disease-resistant and can be grown in lowlands as well as mountain regions [8]. It is frost- and disease-resistant and can be grown in lowlands as well as mountain regions [8] This species has dark-blue, elongated-shape fruits, which contain a high amount of vitamin C, anthocyanins, and flavonoids (quercetin, rutin) [9,10]

Objectives

Methods

Results

Conclusion