Abstract
Abstract In vitro cultivated apple pedicels without a primary abscission layer can form a secondary (adventitious) abscission layer, especially under the influence of auxins.In the apple cv. Cox's Orange Pippin abscission can only be induced by auxins, while the site of the abscission layer, a few millimetres from the basal ends of the pedicels, is fixed and independent of the auxin concentration. The auxin treatment has to last at least 5–6 days to induce abscission, which is not affected by the presence of a flower. A secondary layer does not occur when pedicels are placed inverted on the media. Although abscission occurs both in light and in darkness, it is strongly promoted by light. Abscission is also accelerated by raising the temperature from 9°C to 21–25°C. High concentrations of 2,3,5‐triiodobenzoic acid reduce the percentage of auxin‐induced abscission. Sugar is required, but the presence of macro‐elements is not essential.
Published Version
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