Induction of regulatory T cells by Opisthorchis viverrini

Abstract

Event Abstract Back to Event Induction of regulatory T cells by Opisthorchis viverrini Chamraj Kaewraemruaen1, Rasana W. Sermswan2 and Surasakdi Wongratanacheewin1, 3* 1 Khon Kaen University, Microbiology, Thailand 2 Khon Kaen University, Biochemistry, Thailand 3 Khon Kaen University, Thailand Opisthorchis viverrini is a liver fluke that prevalence in Thailand and neighboring countries. Our previous studies demonstrated that this helminthic infection could induce Th2 and regulatory T cell (T reg)­like cytokines, such as TGF­-β, IL-­4 and IL­-10 in infected hamsters. This study aims to investigate CD4+T cell responses against O. viverrini infection in hamsters. Hamsters were infected with 50 Mc for 10 months. The mononuclear cells of spleen and mesenteric lymph node (MLN) from infected or control groups were stimulated with 10 µg/ml of crude somatic antigen (CSAg) for 24 hrs and stained for CD4, CD25, IL­-4, IL­10, IFN­-γ and TGF-­β, detected by flow cytometer. The IL-­4+ and TGF-­β+ CD4+ T cells in MLN were found significantly higher than control whereas IL-­4+ and IL­-10+ CD4+ T cells were increased in infected spleens. In contrast CD4+IFN­-γ+ T cells in both spleen and MLNs were not significantly different between infected and control groups. Furthermore significant numbers of TGF­-β+CD4+T cells in O. viverrini infected group in MLN were found to be CD25+. The result indicated the induction of IL­4+CD4+ T cells in systemic immune responses whereas TGF-­β+CD4+ T cells are predominated in mucosal immune responses. To confirm the induction of regulatory T cells by parasite, Balb/c mice were immunized with CSAg, antigen-specific T cell clones were established and found to be TGF­-β producing T reg cells. Taken together, our study demonstrated that O. viverrini could stimulate the T reg lead to down regulation of the host immune system making parasite survival. Acknowledgements The Higher Education Research Promotion and National Research University Project of Thailand , Office of the Higher Education Commission, through the Health Cluster (SheP-GMS), Khon Kaen University and Melioidosis Research Center, Khon Kaen University, Thailand References Jittimanee, J., Sermswan, R.W., Puapairoj, A., Maleewong, W., Wongratanacheewin, S., 2007. Cytokine expression in hamsters experimentally infected with Opisthorchis viverrini. Parasite Immunol 29, 159-167. Keywords: regulatory T cells, Opisthorchis viverrini, mucosal immunity, T cell clone, BALB/c Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Kaewraemruaen C, Sermswan RW and Wongratanacheewin S (2013). Induction of regulatory T cells by Opisthorchis viverrini. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00217 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Surasakdi Wongratanacheewin, Khon Kaen University, Microbiology, Khon Kaen, 40002, Thailand, sura_wng@kku.ac.th Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Chamraj Kaewraemruaen Rasana W Sermswan Surasakdi Wongratanacheewin Google Chamraj Kaewraemruaen Rasana W Sermswan Surasakdi Wongratanacheewin Google Scholar Chamraj Kaewraemruaen Rasana W Sermswan Surasakdi Wongratanacheewin PubMed Chamraj Kaewraemruaen Rasana W Sermswan Surasakdi Wongratanacheewin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.