Abstract
One of the most important problems for growing Dendrobium orchids is disease infection resulting in losses of yield and quality of inflorescence. At present, the public has been concerned about chemical residues in environment, human health, and the increasing chemical resistance of pathogens. Therefore, the objective of this experiment was to study the effects of chitosan on plant disease defence and in vitro growth of Dendrobium 'Eia Sakul'. The seedling of Dendrobium 'Eia Sakul' was cultured on Vacin and Went 1949 (VW) medium supplied with the chitosan at the concentrations of 0 (control), 10, 20, 40 and 80 mg.L -1 at 25°C. Chitinase and beta-1,3-glucanase activities; the enzyme associated with plant disease defence, and the growth of Dendrodium were then determined after culturing for 4, 8, 12, 22, 24 and 26 weeks. The results revealed that the activities of chitinase and beta-1,3-glucanase dramatically increased during cultured, and the chitosan treated Dendrobium showed significantly the highest activities of both enzymes on week 22, 24 and 26 when compared with non-treated. However, chitosan treatments were not significant effects on the Dendrobium growth; plant height, number of leaves, length of leaves, width of leaves, number of root, length of root and the number of plant per cluster. Total chlorophyll content in leaves also measured. Cholophyll content of Dendrobium treated with chitosan at 10 and 20 mg.L -1 was significant higher than that of control after culturing for 24 and 26 weeks.
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