Abstract
The authors are interested in determining whether the MN technique can be developed as an in situ assay for genotoxicity in fish species; and in particular, the brown bullhead (Ictalurus nebulosus). In this study, central mudminnows (Umbra limi) and brown bullheads were exposed to EMS and benzo(a)pyrene (BaP) by intraperitoneal injection. The various experiments were designed to: i) compare the sensitivity of the MN and SCE assays using the mudminnow model; ii) determine whether MN are induced in the erythrocytes of the brown bullhead by exposure to genotoxic chemicals (EMS and BaP); iii) determine whether erythrocytes harvested from the erythropoietic tissues of fish (pronephros) have a greater incidence of MN than peripheral erythrocytes; iv) examine the association between the induction of MN and the induction of nuclear abnormalities in erythrocytes after exposure of genotoxic agents.
Published Version
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