Abstract
Addition of the inhibitors of thiol-protease leupeptin and antipain to adult rat hepatocytes in primary culture caused rapid inhibition of α-N-benzoylarginine-β-naphthylamide (BANA) 2 2 Abbreviations: BANA, α-N-benzoyl-DL-arginine-β-naphthylamide; Hb, hemoglobin. -hydrolase and induction of the acid-protease, hemoglobin (Hb)-hydrolase. This induction was inhibited completely by cycloheximide and puromycin, and partially by actinomycin D. Addition of leupeptin did not affect the activity of acid phosphatase, cytochrome c oxidase, or NADPH cytochrome c reductase or protein synthesis. Pepstatin, an inhibitor of acid-protease, did not induce BANA-hydrolase activity. These findings suggest that inhibitors of thiol-protease specifically induced acid-protease in lysosomes by some unknown mechanism.
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