Abstract
3-Chloro-4-(dichloromethyl)-5-hydroxy-2(5 H)-furanone (MX), a potent direct-acting Salmonella mutagen found in chlorinated drinking water, was tested in Chinese hamster ovary (CHO) cells for the induction of mutation at the hypoxanthine phosphoribosyl transferase locus to 6-thioguanine resistance (TG r). MX treatment of CHO cells for 3 h at 37°C resulted in significant dose-related increases in mutant frequency. The lowest observed effective dose was 2.5 μg/ml, where the cloning efficiency estimated on the day after treatment was not affected. The relationship between the dose of MX and the frequency of TG r mutants was approximately linear over the range of 0–5 μg/ml with an estimated slope (±95% confidence limits) of 7.2 ± 2.6 mutants per 10 6 clonable cells per μg/ml.
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