Induction of Epithelial Ovarian Cancer by Implantation of 7,12-dimethylbenz(a)athracene (DMBA) Coated Silk in Rats.

Abstract

Objective: This research aims to get the relevant animal model for epithelial ovarian cancer and ultrasonography approach to detect volume and size of ovarian cancer. Methods: Induction of ovarian cancer in rats was carried out using 7,12-Dimethylbenz [a] anthracene (DMBA) coated on silk suture and then implanted in the left ovary of female Wistar rats. Nodule were determined weekly after the first surgery by palpating the abdominal wall of rats. Utrasonography was carried out at 20th week. Rats were euthanized, ovarian organs were taken and histopathological examination was performed with haematoxylin and eosin staining. Results: Nodules in DMBA-treated rats were noted at 16th weeks post implantation and reaching a cummulative incidence of 96, 4% at 24th weeks. On ultrasound examination at the 20th week, there were significant differences in left and right ovarian size and volume. The histological type of induced tumor included: serous carcinoma cell, clear cell carcinoma, sarcoma and squamous carcinoma. Conclusion: The implanted 7,12-Dimethylbenz[a]anthracene (DMBA) coated silk suture in the ovaries rats can be used as animal model for testing therapeutics agent of ephitelial ovarian cancer and ultrasonography can determine the size and volume of tumors formed in ovary. Key words: 7,12-Dimethylbenz[a]anthracene, Animal model, Epithelial ovarian cancer, Ultrasonography.