Abstract
Abstract The growth requirements of several normal diploid human skin fibroblasts in cell culture were investigated. The human diploid cells ceased growing predominantly due to depletion of serum factors in the medium. Quiescent cultures could be reinitiated to synthesize DNA by the addition of the bovine pituitary factor, fibroblast growth factor (FGF). FGF at saturating concentrations (50 ng/ml) can initiate DNA synthesis in quiescent cultures at approx. 40 to 50% of the efficiency compared with 30% fetal calf serum. Hydrocortisone and insulin did not potentiate the action of FGF in completely quiescent cultures. In the absence of serum FGF, insulin and hydrocortisone failed to maintain continuous cell growth suggesting the involvement of additional serum components. FGF failed to stimulate cells arrested by histidine deprivation to grow.
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