Abstract
1-Methyladenine induces cytoplasmic maturation as revealed by the elevation of fertilization membrane both in oocytes from which germinal vesicles were removed and in fragments of the enucleated oocytes. However, at the most only abortive cleavage occurs in the enucleated oocytes. Thus, fertilizability of oocytes can be achieved with 1-methyladenine treatment but without any participation of germinal vesicle material, though it appears that mixing of germinal vesicle material with egg cytoplasm is a necessary prerequisite for genuine cleavage.
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