Abstract
The effect of injection of various concentrations (4 ng to 0.5 μg/larva) of α-amanitin on chromosomal RNA synthesis in larval salivary glands of D. hydei was investigated at subsequent time intervals (90 min to 20 hrs) after injection. — As revealed by autoradiography, 3H-uridine incorporation into the polytene chromosomes was greatly reduced as compared with that in control larvae. In α-amanitin injected larvae, new chromosome puffs could be induced by a temperature treatment. These puffs never attained a size comparable to that in the controls. The newly induced puffs did not show specific incorporation of 3H-uridine. — Puffs which were active before the toxin was applied undergo a reduction in size and incorporation of 3H-uridine.
Published Version
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