Abstract
In this study, the young leaves of Cassia mimosoides were used as explants. On the basis of 1/2 MS +2.0 mg/L 6-BA +0.5 mg/L NAA medium, we observed the effect of adding different factors on the callus induction of C. mimosoides. The results shown that after 20 days of callus induction, it was found that the addition of 150 mg/L vitamin C (VC) did not affect the induction efficiency, but the growth of callus became slower; and when the concentration was higher than 150 mg/L, the formation of callus would be inhibited. The induction of callus would be restrained while adding different concentrations of Cu2+ into mediums. Moreover, glutamine (Gln) had little effect on the induction of callus. Furthermore, the addition of hydrolyzed casein (CH) would not affect the formation of callus, but the high concentration of CH could affect the growth status of callus. The induction efficiency of callus was severely inhibited by exogenous addition of silver nitrate (AgNO3), and the growth status of callus was poor, and the phenomenons of early rooting had happened at the same time. The results of this study provided a theoretical basis for the subsequent optimization of bud proliferation and rooting in C. mimosoides.
Highlights
Cassia mimosoides, known as Mimosa cassia, is a plant of the Cassia
Different concentrations (0, 150, 300 and 450 mg/L) of vitamin C (VC), different concentrations (0, 5, 10 and 15 mg/L) of CuSO4, different concentrations (0, 10, 20 and 40 mg/L) of L-Glutamine (Gln), different concentrations (0, 200, 400 and 600) of casein hydrolysate (CH) and different concentrations (0, 50, 100 and 200 mg/L) of AgNO3 were added into the mentioned above basic medium for callus induction from leaf explants of C. mimosoides
The concentration of VC in the medium influenced the response of callus induction clearly (Table 1)
Summary
Known as Mimosa cassia, is a plant of the Cassia It was originally published in the book of "Herbal for Relief of Famines" in the Ming Dynasty. It is a homology for medicine and food in China, and mainly distributed in Guangdong, Guangxi, Sichuan, Hunan and Fujian. Some studies shown that due to the high content of flavonoids and phenols in the ethyl acetate extract of C. mimosoides, it had good effect on DPPH (1,1-Diphenyl-2-picrylhydrazyl Free Radical) scavenging ability and strong antioxidant activity, playing a role in protecting the liver [7]. Due to the long natural reproduction cycle and long time to obtain seeds in the natural environment, the development of the tissue culture technology of C. mimosoides could shorten the growth cycle, and provide raw material support for further understanding of the basic research on the physical activity analysis and liver protection application
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