Abstract
Citrus macrophylla is an important citrus rootstock for Mexican lemon (Citrus aurantifolia S.). This study was conducted to select explant type and to optimize cultural requirements for induction callus of C. macrophylla in vitro. The explants tested were leaf, epicotyl, cotyledon, and root segments excised under sterile conditions from 4-week-old nucellar seedlings. The various medium comprising either basal of Murashige and Skoog (MS) or Murashige and Tucker (MT) salts suplemented with various concentrations of plant growth regulators, including naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin) were used for the establishment of the explants. All cultured explants initiated callus from the cut ends after 2 weeks, when cultured on a modified MT medium suplemented with 6 mg NAA and 0.2 mg kinetin; cotyledon segments were the best explant for callus induction and development (43 mm2). Root segments were the lowest explants for callus induction.
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