Abstract
Lamprocapnos spectabilis (L.) Fukuhara is a perennial plant species valued in the horticultural, cosmetic, and pharmaceutical markets. To date, however, there were no studies on tissue culture systems in this species when adjusted from non-meristematic explants. The aim of this study is to induce callogenesis, organogenesis, and somatic embryogenesis in non-meristematic explants of Lamprocapnos spectabilis ‘Alba’ cultured in various media and to analyze the chemical diversity of the produced callus. Leaf, petiole, and internode explants were cultured on the modified Murashige and Skoog (MS) medium fortified with various combinations and concentrations of 6-benzyladenine (BA), indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorphenoxyacetic acid (2,4-D), and picloram (PIC). After 10 weeks of culturing, the morphogenetic response of explants was evaluated and the concentration of chlorophylls, carotenoids, anthocyanins, and polyphenols in callus was analyzed. There was no influence of explant type on the callogenesis efficiency (62.1–65.3%). The highest fresh weight of callus was produced on leaf explants in the presence of 2,4-D or PIC. In contrast, the highest share of dry weight was found in internode-derived calli and cultured on IAA-supplemented medium (up to 30.8%). Only 2.5% of all explants regenerated adventitious shoots, while rhizogenesis was reported in 4.5% of explants. Somatic embryos were produced indirectly by 0% to 100% of explants, depending on the culture medium and explant type. The highest mean number of embryos (11.4 per explant) was found on petioles cultured in the MS medium with 0.5 mg·L−1 BA and 1.0 mg·L−1 PIC. Calli cultured in media with NAA usually contained a higher content of primary and secondary metabolites. There was also a significant impact of explant type on the content of anthocyanins, polyphenols, and carotenoids in callus. Further studies should focus on the elicitation of metabolites production in callus culture systems of the bleeding heart.
Highlights
No callogenesis was reported in the plant growth regulators (PGRs)-free control medium (M0)
The efficiency of caulogenesis was surprisingly low with the bleeding heart, even though media fortified with cytokinins and auxins were successfully utilized with numerous other plant genera [16,22]
The present study showed that metabolite biosynthesis in L. spectabilis can be controlled by medium variables and explant type, which is in agreement with the reports of other authors [18]
Summary
Dicentra spectabilis (L.) Lem.) or bleeding heart is a perennial plant species, originating from Asia. It is a member of a small botanical family known as Fumariaceae, which is closely related to Papaveraceae. Due to its spectacular flowers, the species is commonly used as both an indoor and outdoor/landscape plant [1]. There is lots of information on the health-stimulating properties of extracts derived from the bleeding heart. Its roots have been used in Asian folk medicine for treating pus and paralysis [2]. Strong anti-aging effects, against UV-induced skin photoaging, of L. spectabilis extracts, were observed even at a low (0.1% w/v) concentration [3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
