Abstract
In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus, the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu2+ and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu2+ on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu2+ was 5 mg/L.
Highlights
Anoectochilus formosanus, known as sapphire orchid, is a perennial herb in Orchidaceae
The results shown that different concentrations of DA-6 significantly affected the regeneration efficiency of adventitious buds (Table 1 and Figure 1(A-D))
The highest percentage of regeneration was gained at 2 mg/L of DA-6 among the four concentrations, as reflected by a 93.33% of adventitious buds regeneration rate (Table 1 and Figure 1C)
Summary
Anoectochilus formosanus, known as sapphire orchid, is a perennial herb in Orchidaceae. It is mainly distributed in the cool and humid areas at an altitude of 500-1800 m in Taiwan and Fujian, China [1,2]. A. formosanus has high medicinal value, the main chemical components in A. formosanus are polysaccharides, flavonoids, glycosides, organic acids, steroids, trienes, alkaloids and nucleosides. These compounds have been shown to be the main biologically active substances, with major pharmacological effects such as anti-diabetes, anti-inflammatory, antiinflammatory, anti-viral, liver protection, kidney protection, immunomodulation and anti-tumor [4]. It is especially popular in Taiwan province and is known as the “king of medicine” [5]
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