Abstract
Stahpylococcus epidermidis (S. epidermidis), S. warneri and S. aureus subsp. anaerobious were isolated from sheep abscess. Crude FAME was extracted from supernatant fluid of a 24hrs culture of S. aureus subsp. anaerobious grown in Trypticase Soy Broth at 37°C with continuous agitation. Fourteen heads of sheep were used. Three of them were inoculated with S. epidermidis + FAME and other three were inoculated with S. warneri + FAME. Eight animals were divided into four groups, two animals in each, used as control and inoculated with S. epidermidis, S. warneri, S. aureus subsp. anaerobious and FAME. All animals were inoculated subcutaneously at the neck region. After fourteen to eighteen days, sites inoculated with S. epidermidis + FAME, S. warneri + FAME and S. aureus subsp. anaerobious showed abscesses grossly then confirmed bateriologically and histopathologically, while those inoculated with S. epidermidis alone, S. warneri alone and FAME showed no abscess.
Highlights
Abscess disease in sheep has a great economical importance in Sudan
Inoculation of sheep with treated bacteria resulted in abscess formation (Figure 1 and Figure 2)
The control sites which were injected with S. epidermidis, S. warneri and Fatty Acid Modifying Enzyme (FAME) revealed in no swelling or caseation (Table 1)
Summary
Abscess disease in sheep has a great economical importance in Sudan. For the organism to survive and multiply in the host tissue and causes abscess it must produce an estrifying enzyme known FAME which inactivates the bacteriocidal fatty acids [4,5,7]. Some of them were known to produce FAME but unable to form abscess; from these S. epidermidis and S. warneri (coagulase-negative Staphylococci) are examples [6]. While some CNS strains produced FAME activity comparable to the lab strain of S. aureus, the pattern of FAME activity varied among strains and across species of staphylococci [9]
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