Inducing Adventitious Buds Regeneration from Leaf Explants of Purple Coneflower (Echinacea Purpurea L.)

Abstract

In the present study, purple coneflower leaves were used as explants to explore the effects of basic medium, CuSO4, and Diethyl aminoethyl hexanoate (DA-6) on induction of adventitious buds. The results shown that MS medium was the most suitable basic medium for inducing adventitious buds regeneration from leaf explants. When the concentration of CuSO4 was at 0.5 mg/L, a great effect of induction of adventitious buds was obtained (90%). Meanwhile, the optimal culture condition for the proliferation of adventitious buds was MS medium containing 0.5 mg/L 6-benzylaminopurine (6-BA).