High efficiency adventitious shoot bud formation and plant regeneration from leaf explants of Dianthus chinensis L.

Abstract

Leaf explants of Dianthus chinensis L. cultured on Murashige and Skoog’s (MS) medium with 6-benzylaminopurine (BAP, 3 mg/l)+1 -naphthaleneacetic acid (NAA, 0.5 mg/l) or BAP (3 mg/l)+ NAA (1 mg/l) produced adventitious shoot buds directly on the surface of the leaf explants without formation of intervening callus. Callus formation occurred after the induction of adventitious buds. Leaf explants cultured on BAP (0.5 mg/l) and 2,4-dichlorophenoxyacetic acid (2,4-D, 1 mg/l) supplemented MS medium also produced adventitious shoot buds along with excessive callus formation. Shoot buds formed on BAP and 2,4-D containing medium need to be subcultured on BAP and NAA supplemented medium for elongation, while the shoot buds induced on BAP and NAA supplemented medium elongated in the primary culture itself. Shoots were rooted on half strength MS medium with IAA (0.05 mg/l). Most rooted shoots grew well and produced viable seeds when grown in field conditions. This protocol is useful for clonal micropropagation and for Agrobacterium-mediated gene transfer in D. chinensis. Adventitious shoot bud formation directly from the leaf explants in D. chinensis is reported here for the first time.