Induced oxidative equilibrium damage and reduced toxin synthesis in Fusarium oxysporum f. sp. niveum by secondary metabolites from Bacillus velezensis WB.

Abstract

In this study, the antifungal mechanism of secondary metabolites from the WB strain against Fusarium oxysporum f. sp. niveum (Fon) was investigated. The WB strain induced the accumulation of reactive oxygen species in Fon hyphae and caused morphological changes, including surface subsidence and shrinkage deformation. The cell-free supernatants (CFSs) from WB treatment caused a significant increase in superoxide dismutase, catalase, peroxidase and glutathione reductase activities and the contents of soluble protein and malondialdehyde. Additionally, CFSs from WB decreased the fusaric acid concentration in Fon. Transcriptome analysis revealed that the expression of some antioxidant-related genes was upregulated and that the expression of mycotoxin-related genes was downregulated. Four polypeptide compounds from the WB strain, including iturin A, fengycin, surfactin and bacitracin, were identified by ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry analysis and complete genome mining. RT-qPCR and a quantitative analysis confirmed that the presence of Fon induced the expression of polypeptide genes and elevated polypeptide production. The combined minimum inhibitory concentration and quantitative analysis of four polypeptides revealed that iturin A, fengycin, surfactin and bacitracin might be responsible for inhibiting the growth of Fon. In conclusion, secondary metabolites from strain WB exhibited antifungal effects on Fon by triggering oxidative stress and decreasing toxin levels.