Antioxidant Status of Rat Liver Mitochondria under Conditions of Moderate Hypothermia of Different Duration.

Abstract

For evaluation of the contribution of the antioxidant system of mitochondria into the dynamics of changes in the prooxidant status, the content and activity of some of its components were studied under conditions of moderate hypothermia of varying duration. It was found that short-term hypothermia significantly increased superoxide dismutase activity and decreased the levels of low-molecular-weight antioxidants. Increasing the duration of hypothermia to 1 h led to suppression of activities of superoxide dismutase, glutathione reductase, and glutathione peroxidase and a decrease in glutathione content. Further prolongation of hypothermia (to 3 h) was associated with a significant increase in superoxide dismutase and glutathione peroxidase activities and normalization of the rate of glutathione reductase catalysis; the concentration of glutathione increased significantly.