Induced Lesions by Dimethyl Benzanthracene Subcutaneous Inoculated in Mouse

Abstract

Dimethyl benzanthracene subcutaneous administration in mice leads to appearance of some necrosis zones with about 1 cm diameter at inoculation place. At necropsic examination effected to 15 months from experiment star were observed: necrosis zones and cutaneous punctiform erosions in dorsal region, in animals from lots wit h subcutaneous inoculation with dimethyl benzanthracene (lots 1, 2 and 3); increasing of adi pose tissue layer in animals whose food was with carotenoids (lots 4 and 5). Histopathologically wer e put into evidence the epidermis hyperplasia and hypertrophy in animals of lot with dimethyl benzant hracene subcutaneous inoculation (lot 1); images of dysplasia and methaplasia, changes considered pr eneoplasic. In animals of lots 2 and 3 (with carotenoid supplement in food), histopathologically were observed only small epithelial proliferations as buttons' shape and thickening of dermis papillae basis on fibrous connective tissue. In animals from the lot with subcutaneous dimethyl benzanthracene administration, without carotenoid supplement in food (lot 1), histopathologically app ears esophagus gastric mucous membrane hyperkeratosis, aspect that is not present in case of animals from the lots with carotenoids in food (lots 2, 3, 4 and 5). The protector effect of carot enoids is observed both on skin of inoculated place s with dimethyl benzanthracene and also on esophagus type gastric mucous membrane level. We underline the first time demonstration of protector and healing effect of rodoxanthin.