Abstract
Abstract A cholinesterase inhibition method to detect ihionophosphates (P=S compounds) and their oxygen analogs (P=O) by twodimensional thin layer chromatography is described. Environmental samples are cleaned up on Florex columns and spotted on silica gel thin layer plates. Plates are developed in solvent mixtures of opposite polarity, then sprayed with an enzyme solution and indoxyl acetate. Submicrogram sensitivity is achieved by fluorogenic and chromogenic reaction. Some compounds give characteristic colored reaction products.
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