Abstract
Evidence indicates that dietary I3C decreases serum CH and fat. This study was conducted to examine if luminal I3C would affect the lymphatic absorption of CH and other lipids in rats. Adult male rats with lymph cannulae were infused at 3.0 mL/h for 8 h via a duodenal catheter with a lipid emulsion containing 1.0 mg CH labeled with 14C (14C‐CH), 452.0 μmol triolein, 3.1 μmol α‐tocopherol and 396.0 μmol Na‐taurocholate without (13C‐0) or with 16 mg (I3C‐1) or 40 mg I3C (I3C‐2) in PBS buffer (pH, 6.4). Lymph was collected hourly for 8 h. I3C dose‐dependently lowered the lymphatic absorption of 14C‐CH. The cumulative absorptions of 14C‐CH in rats infused with I3C‐0, I3C‐1 and I3C‐2 were 36.3 ± 4.7, 25.7 ± 3.0 and 9.0 ± 1.9% dose, respectively. Likewise, 13C decreased the lymphatic outputs of total fatty acids and α‐tocopherol in a dose dependent manner. I3C at a high dose (I3C‐2) lowered the lymphatic output of total phospholipids, whereas there was no such effect at a lower dose (I3C‐1). The results indicate that I3C has a profound inhibitory effect on the intestinal absorption of CH and other lipids in rats. Currently, studies are underway to determine whether I3C would affect the absorption of other lipophilic compounds such as persistent organic pesticides.
Published Version
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