Indirect optical trapping using light driven micro-rotors for reconfigurable hydrodynamic manipulation

Unė G Būtaitė,David B Phillips,Jonathan M Taylor,Graham M Gibson,Mike Taverne,Ying-Lung D Ho

doi:10.1038/s41467-019-08968-7

Unė G Būtaitė, David B Phillips + Show 4 more

Open Access

https://doi.org/10.1038/s41467-019-08968-7

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Abstract

Optical tweezers are a highly versatile tool for exploration of the mesoscopic world, permitting non-contact manipulation of nanoscale objects. However, direct illumination with intense lasers restricts their use with live biological specimens, and limits the types of materials that can be trapped. Here we demonstrate an indirect optical trapping platform which circumvents these limitations by using hydrodynamic forces to exert nanoscale-precision control over aqueous particles, without directly illuminating them. Our concept is based on optically actuated micro-robotics: closed-loop control enables highly localised flow-fields to be sculpted by precisely piloting the motion of optically-trapped micro-rotors. We demonstrate 2D trapping of absorbing particles which cannot be directly optically trapped, stabilise the position and orientation of yeast cells, and demonstrate independent control over multiple objects simultaneously. Our work expands the capabilities of optical tweezers platforms, and represents a new paradigm for manipulation of aqueous mesoscopic systems.

Highlights

Optical tweezers are a highly versatile tool for exploration of the mesoscopic world, permitting non-contact manipulation of nanoscale objects
We combine concepts from both optical and hydrodynamic approaches, to create a fully reconfigurable system capable of inducing highly localised flow fields targeted only at specific particles, leaving other objects in the sample largely unperturbed. This system retains the flexibility of optical tweezers, and so can operate anywhere throughout the sample. As it relies on hydrodynamic forces, the platform offers a new route to overcome some key limitations of conventional optical tweezers, namely that many types of target particles cannot be directly optically tweezed[23], and that biological systems can be damaged by the high intensities of tightly focused laser beams[24,25]
Our technique relies on a simple principle: when an optically trapped micro-structure is moved, it displaces pico-litre quantities of the surrounding fluid in a highly predictable manner, exerting well-defined hydrodynamic forces on nearby objects[26]

Summary

Introduction

Optical tweezers are a highly versatile tool for exploration of the mesoscopic world, permitting non-contact manipulation of nanoscale objects. We combine concepts from both optical and hydrodynamic approaches, to create a fully reconfigurable system capable of inducing highly localised flow fields targeted only at specific particles, leaving other objects in the sample largely unperturbed This system retains the flexibility of optical tweezers, and so can operate anywhere throughout the sample. As it relies on hydrodynamic forces, the platform offers a new route to overcome some key limitations of conventional optical tweezers, namely that many types of target particles cannot be directly optically tweezed[23], and that biological systems can be damaged by the high intensities of tightly focused laser beams[24,25]. The reconfigurable nature of this technique opens up a variety of new experimental paradigms, such as the ability of micro-rotors to rearrange around, and move along with the particles they are steering—akin to hydrodynamic tweezing with a dynamically reconfigurable microfluidic chip

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