Abstract
Hancornia speciosa Gomes is a fruit species belonging to the Apocynaceae family and holds social, cultural and economic potential mostly due to its use in composition of many food industry products and the consumption its fruits in natura . Several aspects regarding their propagation need further studies, since the species is undergoing a continuous process of domestication. The objective was to obtain an in vitro protocol for indirect organogenesis and rooting with subsequent acclimatization of H. speciosa plants. To obtain indirect organogenesis, internodal segments were inoculated in WPM culture medium gelled with 7 g L -1 agar, added with 30 g L -1 sucrose, 0.4 g L -1 PVP, and supplied with different concentrations of 2,4-D (0.0; 2.46; 7.38; 12.30; and 17.22 µM), BAP (0.0; 4.92; 9.84; 14.76; and 19.68 µM), and TDZ (0.0; 4.92; 9.84; 14.76; and 19.68 µM). For the in vitro rooting, shootings with approximately 6.0 cm diameter length kept for 15 days in WPM medium with no plant growth regulator and, afterwards, subjected to treatments with different auxins (Control; 9.84 µM IBA; 9.84 µM NAA; and 9.84 µM IAA) as well as the combination among them, in order to verify their effects on percentage of rooting (%), number of roots, and average length of the largest root (cm). The formation of calluses was observed in all explants subjected to the concentrations of the regulators tested. The highest shooting regeneration occurred with 7.38 µM 2,4-D (73%). The highest percentage of shoot rooting (80%) and roots with the largest length (1.3 cm) were found in the culture medium with the combination of 4.92 µM NAA and 4.92 µM IBA. The in vitro regeneration of H. speciosa is feasible. The acclimatization of rooted shoots in Trospstrato® was accomplished with successful and 100% survival of plant material was observed during this stage.
Highlights
The production of fruit stands out in agriculture worldwide mostly due to economic and social importance creating jobs and moving the economy (SANTOS FILHO et al, 2014)
For the in vitro rooting, shootings with approximately 6.0 cm diameter length kept for 15 days in Wood Plant Medium (WPM) medium with no plant growth regulator and, afterwards, subjected to treatments with different auxins (Control; 9.84 μM indole-3-butyric acid (IBA); 9.84 μM naphthalaeneacetic acid (NAA); and 9.84 μM indole-3-acetic acid (IAA)) as well as the combination among them, in order to verify their effects on percentage of rooting (%), number of roots, and average length of the largest root
It was noteworthy that the highest callus formation occurred in the presence of growth regulators when compared to their absence in the culture medium (Figures 1A, 1B, and 1C)
Summary
The production of fruit stands out in agriculture worldwide mostly due to economic and social importance creating jobs and moving the economy (SANTOS FILHO et al, 2014). Even though Brazil is among the main fruit producers in the world, several native fruit species are poorly commercialized in the country which is result of the few technified plantations intended to large-scale production. These species are extracted by local communities mostly because of the high nutritive value of their fruits (SILVA JÚNIOR, 2004). Among the several species with commercial exploitation potential, Hancornia speciosa Gomes popularly known as ‘mangabeira’ is noteworthy It has a wide geographic distribution covering the territory from the State of Amapá to the State of São Paulo (MACHADO et al, 2004). Considering the scarcity of works related to the organogenesis of H. speciosa, the present work aimed the establishment of a micropropagation protocol via indirect organogenesis in order to provide regenerated shoots to the rooting and acclimatization stages
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