Abstract
An indirect method for the determination of ca. 10 −4 M amino acids is described which is based on the reaction of the latter with 2,4,6-trinitrobenzene-1-sulphonic acid at pH 11.7 and the measurement of the S 2 emission in a hydrogen-nitrogen flame that is produced from the resulting compound after the addition of hydrochloric acid. The optimum conditions for the determination of amino acids were investigated. The amino acids examined were glycine, alanine, valine, methionine, serine, threonine and histidine.
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