Abstract
A total of 1137 tissue culture banana (Musa spp.) seedlings were collected between May and July 2007 from nurseries of the Thika National Horticulture Research Centre of the Kenyan Agricultural Research Institute (KARI), Jomo Kenyatta University of Applied Technology and Agro-Genetic Technologies. The seedlings were approximately 3 months old, since potting. Leaf samples from the seedlings were indexed for Banana streak virus (BSV) at KARI-Kabete laboratory. Immuno-capture PCR specific primers to BSV-GF and BSV-Cav were used. Of the 1137 seedlings indexed, 120, 175, 112, 200, 130, 150, 100, 75 and 75 were of cultivar 'Dwarf Chinese' (AAA genome), 'FHIA 18' (AAAB genome), 'Solio' (AAA-EA genome), 'FHIA 17' (AAAA genome), 'Nusu Ng'ombe' (AAA-EA genome), 'Grand Naine' (AAA genome), 'Williams' (AAA genome), 'Uganda Green' (AAA genome) and 'Chinese Cavendish' (AAA genome), respectively. A total of 265 samples from the nine cultivars tested positive for BSV. 'Dwarf Chinese', 'Grand Naine' and 'Williams' cultivars tested positive both for BSV-Cav and BSV-GF. The PCR protocol for detection of BSV was validated. Validation tests were conducted, utilizing fresh banana leaf tissue samples from cultivars 'Lisulya' and 'Khabusi Mboki' (AAA-EA genome), greenhouse tissue cultured plantlets of 'Dwarf Chinese' and 'Ex-Rongai' (AAA genome), a positive control, consisting of freeze-dried banana leaves from Queensland University of Technology (QUT), and a PCR positive control plasmid DNA sample, also from QUT. Extraction of DNA was done using Qiagen DNeasy plant mini kit to generate a DNA template for PCR amplification.
Published Version
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