Abstract
Objective: The purpose of this paper is to present a case study on how a recently proposed reproducibility framework named Environment Code-First (ECF) based on the Infrastructure-as-Code approach can improve the implementation and reproduction of computing environments by reducing complexity and manual intervention. Methodology: The study compares the manual way of implementing a pipeline and the automated method proposed by the ECF framework, showing real metrics regarding time consumption, efforts, manual intervention, and platform agnosticism. It details the steps needed to implement the computational environment of a bioinformatics pipeline named MetaWorks from the perspective of the scientist who owns the research work. Also, we present the steps taken to recreate the environment from the point of view of one who wants to reproduce the published results of a research work. Findings and Conclusion: The results demonstrate considerable benefits in adopting the ECF framework, particularly in maintaining the same applicational behavior across different machines. Such empirical evidence underscores the significance of reducing manual intervention, as it ensures the consistent recreation of the environment as many times as needed, especially by non-original researchers. Originality/Value: Verifying published findings in bioinformatics through independent validation is challenging, mainly when accounting for differences in software and hardware to recreate computational environments. Reproducing a computational environment that closely mimics the original proves intricate and demands a significant investment of time. This study contributes to educate and assist researchers in enhancing the reproducibility of their work by creating self-contained computational environments that are highly reproducible, isolated, portable, and platform-agnostic.
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