Increasing the Production of L-asparaginase from Bacillus substilis RRM-1 by UV-Mutation

Abstract

L-asparaginase is an enzyme that can hydrolyze L-asparagine to L-glutamic acid and ammonia. This enzyme has essential usage for food and health. Bacillus subtilis RRM-1 produces enzyme L-asparaginase, yet the production of L-asparaginase from B. substilis RRM-1 still relatively low. Hence, the effort to increase the production of the enzyme is needed. For increasing the enzyme production, the bacteria were mutated using UV rays to increase the production of L-asparaginase. UV rays (15 watts) were exposed to bacteria with a distance of 15 cm and a contact duration of 30, 60, 90, 120 min. Survival rates of bacteria were calculated, and mutant bacteria were analyzed for their ability to produce L-asparaginase. The mutants examined were taken from a 120-minute mutation treatment, which produced six isolates (UV1, UV2, UV3, UV4, UV5, UV6). Mutant UV1 and UV6 showed better results in producing the enzyme L-asparaginase. Moreover, UV6 produces an activity of 8.02 ± 1.22 U/ml. UV6 production is higher than that of B. subtilis RM-1 wild type (3.18 ± 0.65 U/ml). Mutations with UV can produce mutants that can increase enzyme production by three times.