Abstract
An effort has been made to find the best medium and operating conditions for the production of extracellular L-asparaginase by Aspergillus terreus in shake culture fermentation through stepwise optimization strategies like studies on the effect of pH and temperature on L-asparaginase, studies on the enzyme activity in various concentration of L-glutamine and study the growth kinetics for L-glutamine medium. Our study shows that L- glutamine combined with L-asparagine influence the asparaginase production level and physical parameter such as pH and temperature has more contribution in enzyme production. The pH 8 and temperature 35°C found to be good environmental condition the production of extracellular L-asparaginase by Aspergillus terreus.
Highlights
L-asparaginase (L-asparagine amidohydrolase; EC.3.5.1.1) catalyzes the deamidation of L-asparagine to L-aspartic acid and ammonia
L-asparaginase is produced by a large number of micro-organism include E. coli [1], Erwinia cartovora, Enterobacter aerogenes [2], Cornybacterium glutamicum, Candida utilis [3], Staphylococcus aureus [4], Thermus thermophilus [5], Pisum sativum
The filamentous fungus selected to use throughout this study is Aspergillus terreus MTCC 1782 was obtained from Institute of Microbial Technology, Chandigarh, India
Summary
L-asparaginase (L-asparagine amidohydrolase; EC.3.5.1.1) catalyzes the deamidation of L-asparagine to L-aspartic acid and ammonia. L-asparaginase is produced by a large number of micro-organism include E. coli [1], Erwinia cartovora, Enterobacter aerogenes [2], Cornybacterium glutamicum, Candida utilis [3], Staphylococcus aureus [4], Thermus thermophilus [5], Pisum sativum. Clementi in 1922 had reported its presence in guinea-pig serum, the anti-tumour properties of the enzyme were only recognized some time later. Tsuji first reported Deamidation of L-asparagine by extracts of E. coli in 1957. Broome in 1961 discovered that the regression of lymph sarcoma transplants in mice treated with guinea-pig serum was due to the nutritional dependence of the malignant cells on exogenous L- asparagines
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
