Abstract
Objective: Oxidative stress may play an important role in the pathogenesis of Alzheimer's disease (AD). Design and methods: To investigate the possible role of oxidative DNA damage in the pathogenesis of AD, we measured the metabolite concentrations of oxidized nucleosides (pseudouridine, 1-methyladenosine, 5-methylcytidine, 5-methyl-2′-deoxycytidine, 3-methyluridine, N 2, N 2-dimethylguanosine, 8-hydroxy-2′-deoxyguanosine, 5-deoxyadenosine and 2-deoxyguanosine) in urine between AD ( n = 36) and control subjects ( n = 34) using liquid chromatography-mass spectrometry (LC-MS) without urine preparation. Results: In AD, the 3-methyluridine, 1-methyladenosine, 8-hydroxy-2′-deoxyguanosine ( p < 0.05, respectively), 2-deoxyguanosine ( p < 0.01) and pseudouridine, N 2, N 2-dimethylguanosine ( p < 0.001, respectively) were significantly increased when compared with the control subjects. Conclusion: The results indicate that oxidized urinary nucleosides may be useful as biomarkers for AD in early stages.
Published Version
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